pCDH-CMV-CD19 puro

LOCUS      pCDH-CMV-CD19 puro               9066 bp ds-DNA     circular SYN 24-FEB-2023
DEFINITION  Express CD19.
ACCESSION   .
VERSION     .
KEYWORDS    pCDH-CMV-CD19 puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  TITLE     2023 Manel lab plasmids
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4020..4231
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4244..4512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4539..5138
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4539..4558)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5035..5055
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5145..5733
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5198..5218)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5616..5627)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5807..6040
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6112..6233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6149..6168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6203..6222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6273..6408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6335..6354
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6441..6457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6441..6457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6454..6476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6465..6481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6489..6519)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6534..6555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6672..6689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6843..7431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6923..6942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7602..8462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8225..8244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8463..8567)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8635..8653
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8691..8713)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8813..8832
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9026..9048
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9040..9057
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9041..9057
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcgc caccatgcca cctcctcgcc tcctcttctt cctcctcttc
     2341 ctcaccccca tggaagtcag gcccgaggaa cctctagtgg tgaaggtgga agagggagat
     2401 aacgctgtgc tgcagtgcct caaggggacc tcagatggcc ccactcagca gctgacctgg
     2461 tctcgggagt ccccgcttaa acccttctta aaactcagcc tggggctgcc aggcctggga
     2521 atccacatga ggcccctggc catctggctt ttcatcttca acgtctctca acagatgggg
     2581 ggcttctacc tgtgccagcc ggggcccccc tctgagaagg cctggcagcc tggctggaca
     2641 gtcaatgtgg agggcagcgg ggagctgttc cggtggaatg tttcggacct aggtggcctg
     2701 ggctgtggcc tgaagaacag gtcctcagag ggccccagct ccccttccgg gaagctcatg
     2761 agccccaagc tgtatgtgtg ggccaaagac cgccctgaga tctgggaggg agagcctccg
     2821 tgtctcccac cgagggacag cctgaaccag agcctcagcc aggacctcac catggcccct
     2881 ggctccacac tctggctgtc ctgtggggta ccccctgact ctgtgtccag gggccccctc
     2941 tcctggaccc atgtgcaccc caaggggcct aagtcattgc tgagcctaga gctgaaggac
     3001 gatcgcccgg ccagagatat gtgggtaatg gagacgggtc tgttgttgcc ccgggccaca
     3061 gctcaagacg ctggaaagta ttattgtcac cgtggcaacc tgaccatgtc attccacctg
     3121 gagatcactg ctcggccagt actatggcac tggctgctga ggactggtgg ctggaaggtc
     3181 tcagctgtga ctttggctta tctgatcttc tgcctgtgtt cccttgtggg cattcttcat
     3241 cttcaaagag ccctggtcct gaggaggaaa agaaagcgaa tgactgaccc caccaggaga
     3301 ttcttcaaag tgacgcctcc cccaggaagc gggccccaga accagtacgg gaacgtgctg
     3361 tctctcccca cacccacctc aggcctcgga cgcgcccagc gttgggccgc aggcctgggg
     3421 ggcactgccc cgtcttatgg aaacccgagc agcgacgtcc aggcggatgg agccttgggg
     3481 tcccggagcc cgccgggagt gggcccagaa gaagaggaag gggagggcta tgaggaacct
     3541 gacagtgagg aggactccga gttctatgag aacgactcca accttgggca ggaccagctc
     3601 tcccaggatg gcagcggcta cgagaaccct gaggatgagc ccctgggtcc tgaggatgaa
     3661 gactccttct ccaacgctga gtcttatgag aacgaggatg aagagctgac ccagccggtc
     3721 gccaggacaa tggacttcct gagccctcat gggtcagcct gggaccccag ccgggaagca
     3781 acctccctgg cagggtccca gtcctatgag gatatgagag gaatcctgta tgcagccccc
     3841 cagctccgct ccattcgggg ccagcctgga cccaatcatg aggaagatgc agactcttat
     3901 gagaacatgg ataatcccga tgggccagac ccagcctggg gaggaggggg ccgcatgggc
     3961 acctggagca ccaggtgagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     4021 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     4081 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     4141 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     4201 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     4261 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     4321 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     4381 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     4441 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     4501 ccgttacaga tccaagctgt gaccggcgcc tacgctagat gaccgagtac aagcccacgg
     4561 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4621 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4681 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4741 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4801 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4861 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4921 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4981 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     5041 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     5101 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt
     5161 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5221 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5281 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5341 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5401 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5461 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5521 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     5581 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5641 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5701 cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac
     5761 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5821 cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     5881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     6061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     6121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6241 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     6421 gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6481 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6541 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6601 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6661 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7261 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7501 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8281 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8341 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8401 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8461 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8521 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8581 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     8641 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     8701 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     8761 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     8821 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     8881 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8941 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     9001 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     9061 aagctg
//